Comparative Study for the Effect of Gibberellic acid, Kinetin and Indole-3-acetic acid on Seed Germination performance of Dianthus caryophyllus

Seed germination is the major limiting factor for large-scale production and cultivation of crop species. Such attribute also positively as well as negatively affected by some potent plant growth regulators and other chemical compounds. For this, present experiment was undertaken with an objective to investigate the comparison of the effect of various concentrations of plant growth regulators like Gibberellic Acid (GA3), Kinetin and Indole-3-acetic acid (IAA) on seed germination of Dianthus caryophyllus or Carnation. Dianthus seeds were soaked in different concentrations (0 ppm or control, 10, 20, 30 and 40 ppm) of each of GA3, Kinetin and IAA for 24 h at room temperature (25 ± 2°C). Three replicates of each treatment with ten seeds per replicate were arranged for precise physiological analysis. Significant variation was found in all aspects after analysis of variance (ANOVA) of each mean value. After two weeks of seed soaking, it was noted that germination percentages were significantly accelerated by lower concentrations (10 and 20 ppm) of used hormones. Amongst the three potential growth regulators, 20 ppm was found most effective because it showed highest germination percentage for GA3 (87.46%), Kinetin (78.92%) and IAA (75.35%). A great deal of information relating to seed germination practices shows that these plant growth regulators were efficient to overcoming dormancy leading to rapid seed germination. GA3 was selected as best hormone, in this study, which showed highest seed germination. These results could useful to large-scale cultivation of Dianthus caryophyllus plants for improving its floricultural impact worldwide.