HPLC METHOD FOR ESTIMATION OF GALLIC ACID AND EMBELIN IN AVIPATHI CHOORNAM- AN AYURVEDIC FORMULATION

In the present study, an attempt has been made to develop an analytical method for the simultaneous estimation of gallic acid and embelin in marketed Ayurvedic polyherbal formulation Avipathi choornam by High Performance Liquid Chromatography (HPLC). The chromatographic separation was performed on Hemochrom C18 column (250 mm L × 4.6 mm ID column: packing size- 5 μm) with a mobile phase methanol: phosphate buffer pH 3.3 (adjusted with glacial acetic acid) in 75:25 V/V proportion at flow rate of 0.8 mL/min. The analysis was carried out in the absorbance mode at 291 nm. Gallic acid and embelin were satisfactorily resolved with Rt values at 2.36 min and 6.67 min, respectively. The developed method was found to be simple, precise and accurate and can be useful for the quality control of raw material as well as formulations containing these phytoconstituents.