Clindamycin Resistance among Staphylococcus aureus Clinical Isolates in Alexandria
Journal: International Journal of Advanced Microbiology and Health Research (IJAMHR) (Vol.2, No. 1)Publication Date: 2018-03-13
Authors : Ahmed Gaballah Abeer Ghazal;
Page : 24-35
Keywords : ;
- Detection of Mex Efflux Pumps in Pseudomonas aeruginosa using a Multiplex Phenotypic and Genotypic Procedure in an Iranian Referral Hospital
- PHENOTYPIC-GENOTYPIC FEATURES OF MDR PSEUDOMONAS AERUGINOSA AND ACINETOBACTER BAUMANNII FROM DHAKA, BANGLADESH
- Phenotypic and Genotypic Characterization and the Study of Type III Secretion System of Pseudomonas aeruginosa from Surat Region
- Emergence of Antibiotic Resistance and Correlation with the Efflux Pump in Pseudomonas aeruginosa Isolated from Abidjan Hospital
- Direct multiplex recombinase polymerase amplification for rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa in food
Abstract
Background: Macrolides, lincosamides, streptogramins-B (MLSB) resistance can be constitutive or inducible.Staphylococcal strains with constitutive MLSB (cMLSB) phenotype are easily detected using routine disk diffusion technique. Contrarily, inducible MLSB (iMLSB) phenotypeneeds a special double disk diffusion test, D-testto be detected. Misdiagnosis of iMLSB can lead to therapy failure with clindamycin. The aim of this study was to detect the prevalence of erythromycin and clindamycin resistance among Staphylococcus aureus clinical isolates and to detect the resistance encoding genes ermA and ermC. Materials and Methods: One hundred non-repetitive S. aureus isolates were included in this study. Antimicrobial susceptibility was detected to all isolates. The molecular identification of S. aureus and methicillin resistance was performed by detecting femA and mecA genes respectively. iMLSB was detected by D-test assay. The presence of ermA and ermC genes was detected by PCR. Results: Methicillin resistance was detected in 54% of isolates. Most of isolates (73%) were susceptible to erythromycin and clindamycin. Out of the 27 isolates resistant to erythromycin, 10(37%) showed cMLSB phenotype. The remaining 17 (63%) isolates resistant to erythromycin but susceptible to clindamycin were clindamycin were classified into 14 (82%) iMLSB resistance phenotype that showed positive D-test and 3 (18%) macrolide-streptogramin (MSB) resistance phenotype that showed negative D-test. ermA and ermC were detected among the 27 S. aureus isolates resistant to clindamycin and/or erythromycin. Both genes were detected together in 2 isolates (7.5%), ermA was detected alone in 5 isolates (18.5 %) and ermC was detected alone in 20 isolates (74%). Conclusion: From the previous results, we can conclude that D test is a simple, reliable method to detect clindamycin resistance in erythromycin resistant isolates. Nevertheless, the incidence of true susceptibility to clindamycin among erythromycin resistant S. aureus isolates is low.
Other Latest Articles
- Existing organizational culture typologies and organizational commitment at a selected higher education institution in South Africa
- Effects of ambiguity in market reaction to changes in stock recommendations
- Financing losses from natural and man-made disasters by use of crowdfunding
- Effect of Various Substrate and Process Parameters on the Production of Prodigiosin by Serratia marcescens MTCC 4822
- An Introduction to Spatial Database, Spatial Data Types and Spatial Index Analysis using Multidimensional Geo-Database
Last modified: 2018-03-14 21:09:47