Development and Validation of Analysis Methods of Captopril in Tablets with Methods of Area under Curves and Absorbance by Ultraviolet-Visible Spectrophotometry

Development and validation of analytical methods of captopril tablets have been carried out by absorbance method and area under the curve method by UV-Vis spectrophotometry. This study used absorbance calculation principle and area under the curve obtained from measurement of analytical solution using UV-Vis spectrophotometer and distilled water as solvent with several reagents. This reagent is used to oxidize captopril compounds having thiol (-SH) groups in their structure. Captopril linearity was obtained in the concentration range 20 - 60 μg/mL. Correlation coefficient value with absorbance method and area under the curve method are 0.9998 and 0.9994 respectively. The results showed that the sample rate obtained by absorbance method and area under the curve method were 104.55 % ± 0.433 and 92.22 % ± 0.351 for patent tablets; 93.42 % ± 1.306 and 91.09 % ± 0.093 for generic tablets. The average per cent of recovery obtained by absorbance method and area under the curve method were 95.62 % ± 14.444 and 101.55% ± 15.989 for patent tablets; 95.33 % ± 14.278 and 100.96 % ± 15.655 for generic tablets.