Analysis of Genetic Impurity of An Original Cultivar Duku (Lansium parasiticum (Osbeck.) K.C. Sahni & Bennet.), from Jambi, Indonesia Using ITS and MatK Gene

Duku Kumpeh is a original cultivar of Duku (Lansium parasiticum) from Kumpeh a local village in the Jambi, Indonesia. The understanding about genetic information is very important for sustainability used of this prospoctive germplasm of tropical fruit. Identification molecular is very essential to distinguish duku kumpeh with other cultivars of duku in Indonesia. Molecular characteristic of sixteen accessions of Duku Kumpeh were clarified using ITS and MatK gene. DNA from sixteen accessions duku from Jambi were extracted using Genomic KIT plant and amplified them using primer of ITS and MatK gene. The results of amplification DNA samples using both of primer ITS and MatK gene indicated that all of fifteen samples were effectively amplified. So this both of two genes are potential to use for barcoding DNA Duku. Six haplotype of ITS gene and eleven haplotype of MatK gene were identified. The accessions from kumpeh were have different haplotypes. There were genetic impurity in accessions of duku kumpeh. Genetic study and selection of duku kumpeh accessions with superior quality and similar genetic composition were needed in the future.