Validation of method for determining the isoelectric point of protein solutions

This paper presents the validation of a method for determining the isoelectric point of collagen hydrolysates used to obtain biomaterials for medical use produced in the Collagen Department of INCDTP - Division ICPI. The advantage of the presented method is that it is easy to apply, as the Zeta potential is the key parameter controlling electrostatic interactions in particle dispersions. The technique used to measure particle movement velocity in the Malvern Zetasizer Nano instrument series is Laser Doppler Velocimetry (LDV). It is an accepted method for measuring electrophoretic mobility of particles in the solution and by calculating the Zeta potential of protein solutions through extrapolation, the isoelectric point of the protein solution is determined.