ESTIMATION OF XIPAMIDE BY USING HPLC IN PURE AND PHARMACEUTICAL DOSAGE FORM

A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Xipamide in its pure form as well as in tablet dosage form. Chromatography was carried out on Symmetry C18 (4.6×150mm, 5µ) column using a mixture of Acetonitrile:Water(50:50v/v)as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 245nm. The retention time of the Xipamide was 2.4 ±0.02min. The method produce linear responses in the concentration range of 10-50µg/ml of Xipamide. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations. Keywords: Xipamide, RP-HPLC, validation.