ANTECEDENTS OF DENGUE VIRUS DURING THE CLINICAL INVESTIGATIONS IN FAISALABAD

Background: Dengue is the most important viral mutagenic disease in public health, caused by one of the four serotypes (DEN-1, 2, 3 and 4) of the dengue virus, a positive chain RNA virus of the Flaviviridae family, which produces a spectrum of illness ranging from dengue fever to dengue hemorrhagic fever / shock syndrome (shock) due to dengue, the latter a serious infection with vascular and haemostatic abnormality that can lead to death. Objectives: To identify by reverse transcription-polymerase chain reaction (RT-PCR) and specific restriction sites - polymerase chain reaction (RSS-PCR) to the causative agent of the epidemic outbreak presented in the district of Faisalabad in April 2017. Materials and methods: twenty serum samples collected during the dengue outbreak were processed by RT-PCR to determine the serotype; this technique was performed in one step. The RSS-PCR technique was then applied to identify the circulating genotype and the results were subsequently corroborated with viral isolation and sequencing. Results: The analysis of the RTPCR of the RNA extracted from the samples presented an amplified product of 290pb corresponding to the dengue serotype. The analysis of the RSS-PCR products of RNA extracted from dengue isolates corresponded to pattern C, included in genotype III. The isolations of the dengue virus in C6 / 36 cell lines, typed by IFI and the genetic sequencing confirmed the results obtained by the tests previously described. Conclusion: During the classic dengue epidemic outbreak in Lima, genotype III of the dengue virus circulated. Keywords: Classic dengue outbreak; dengue virus; molecular typing; RT-PCR; RSS-PCR.