STUDIES ON QUALITATIVE PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF Piper nigrum

The Piper nigrum L. plant materials such as [leaf and seed] were collected and allowed to shade dry to remove moisture content. The dried samples were used for further studies. The powdered plant materials were filled separately in the thimble and extracted successively using a soxhlet extractor with distilled water, acetone, chloroform, DMSO, ethanol and aqueous. All the extracts were subjected to systematic phytochemical screening for the presence of phytochemical constituents. Carbohydrates, Amino Acids, Proteins, Chloride, Alkaloids, Tannins, Phlobatannins, Steroids, Phenolic compounds, saponins are traced. Antimicrobial activity of the plant extracts were tested by agar well diffusion method against four bacterial pathogens [two Gram positive and two Gram negative strains] such as E. coli, K. pneumoniae, B. cereus and Staphylococcus aureus. In this assay, the leaf and seed of Piper nigrum extracts showed inhibition activities on Gram negative bacterium such as E. coli and K. pneumoniae. The aqueous extract showed inhibitory activity only on Gram negative bacterium B. cereus and S. aureus. This present study the qualitative phytochemical analysis and antibacterial activity were analysed. Key words: Soxhlet extractor, Piper nigrum L. and Antibacterial activity