PREPARATION OF ENZYME BASED STRIPS THROUGH IMMOBILIZATION FOR CALORIMETRIC DETERMINATION OF URIC ACID

The research was carried out for the development of enzyme based uric acid strips for improved diagnosis of diseases. Materials and methods: In this study, the standard curve for uric acid and hydrogen peroxide by direct and indirect method were developed. The enzyme was immobilized on nitrocellulose membrane and filter paper. Enzyme immobilization was done by chemical method, bovine serum albumen (BSA) and gluteraldehyde (cross linker) and bovine serum albumen (BSA) and gluteraldehyde in combination. For optimization of pH 20mM potassium phosphate buffer of different pH (6, 6.5, 7, 8, 9, 10) were prepared. To determine the effect of temperature on strips storage, immobilized strips were stored at 4ºC and at room temperature and used for analysis after every 24 hours. Results: In Dip method, enzymes immobilized nitro cellulose membrane was dipped in 30µl of 18mM uric acid. A clear, visible, bright pink color was developed after 30 minutes in strips. Same results were obtained by pouring the sample on the prepared strips of nitro cellulose membrane and filter paper as obtained by dipping the prepared strips in sample. Conclusion: From this study, it was concluded that more reliable, self-developed and easy to use uric acid test strips were successfully made. Keywords: Uric Acid; Enzyme Based Strips, Biomarker; optimization; diseases