SERODIAGNOSIS OF STRONGYLOIDIASIS WITH THE HELP OF ELISA

Objective: Evaluation of an enzyme- related immunosorbent assay using filariform larvae of Strongyloidesstercoralis is to identify particular antibodies in patients with strongyloidiasis. Design: Source of Serum samples collection was people infected, with strongyloidiasis, with parasitic diseases and common people. By using crude antigen of filariform larvae, its analysis was conducted with the help of IgG-ELISA. Materials and Methods: The study was conducted at the Nishtar Hospital Multan. Subjects: 46 individuals infected with strongyloidiasis is were selected for serum sample collection. 379 cases infected with different parasitic infections and 37 ordinary people were also part of sample collection. Main outcome measures: Positive and negative predictive values, specificity and the sensitivity of the test. Result: The cut-off point value was noted as 0.537. The specificity of the test was recorded as 96.15% while test sensitivity was calculated as 93.47%. The positive predictive value was 72.88% however; negative predictive value was 99.25%. Twelve individuals with toxocariasis, three individuals with hydatidos is and one individual with ascariasis, had antibodies which were reactive in nature against larval antigen. Conclusion: A specific and sensitive diagnostic assay when ELISA method is used with filariform larval antigen. Key Words: Strongyloidesstercoralis, Strongyloidiasis, IgG-ELISA.