AN EFFICIENT METHOD FOR THE ISOLATION OF PUREST FORM OF DNA FROM THE OKRA PLANT

Abelmoschusenculentus (L.) does not have only great nutritional value but also possess some pharmacological activities. This plant is useful for the treatment of genito-urinary disorders, chronic dysentery, curing ulcers and gave relief from hemorrhoids. There are no significant levels of an anti-nutritional factor or endogenous toxins reported in this plant to date. In this study,a new method developed to isolate the purest form DNA from the okra leaves. The young leaves of Okra were collected into the centrifuge tube and several steps have taken to isolate the DNA from the okra plant leaves. It is difficult to isolate genomic DNA from the green leaves of the okra because the tissue of okra contains high mucilaginous acidic polysaccharides content. The DNA isolated by this method was tested for yield, purity, and ability to be amplified by PCR. In this experiment sodium citrate was used for the removal of mucilaginous acidic polysaccharides contain in Okra leaves. these give best result in the highest yields of total genomic DNA.