AN ANTIBIOGRAM STUDY OF SALMONELLA AND E.COLI ISOLATED FROM BROILERS FARMS IN AL GUBA AND SHAHAT

In the present study, the isolation of salmonella and E.coli and their antibiogram patterns in broiler farms in Alguba and Shahat were performed. Tissue samples including (liver, heart blood, spleen, exudates air sac, and intestine) were collected from 60 fresh dead or sick chickens obtained from five and three broiler farms located in Shahat and Alguba, respectively. The isolation and the identification Salmonella and E.coli were performed according to standard methods and the resistance to 13 antimicrobial agents was determined by using Kirby-Bauer disk diffusion method. In the results, E.coli and Salmonella were isolated at percentage of 21.6% and 11.6% respectively. Resistance pattern of Salmonella in descending order was respectively Amoxicillin (100%), Ampicillin(100%), Erythromycin (100%), Doxycycline (50%), Oxytetracyclin (50%), Tetracycline (50%), and Spectinomycin (25%). Whereas E.coli isolates were 100% resistant to Amoxicillin, Ampicillin, Tetracycline, Erythromycin, followed by Enrofloxacin (80%), Oxytetracyclin (80%), Streptomycin (80%), Doxycycline (80%), Sulphamethoxazole (40%), Spectinomycin (60%), and Neomycin (60%).Also in this study, all of salmonella isolates were sensitive to Gentamycin or Colistin sulphate. sensitive pattern of E.coli was recorded to Colistin, followed by Sulphamethoxazole, and Spectinomycin. Moreover, all the isolates of Salmonella and E.coli showed multiple antimicrobial resistances. It is concluded that the rational use of antibiotics broilers farming system should be applied in order to prevent the emergence of antimicrobial resistance pathogens. Additionally, Colistin could be the drug of choice to treat the infection with Salmonellaand E.coli.