Seroprevalence of Mycoplasma gallisepticum in Broiler Chicken by ELISA

Background: This study aimed to determine the sero-prevalence of Mycoplasma gallisepticum (MG) which causes chronic respiratory disease in poultry. It causes huge economic loss to the poultry industry. Materials and Methods: A total of 464 sera samples were collected and tested ELISA test using commercially Mycoplasma gallisepticum ELISA kit (Idexx, USA) to detect the presence of antibodies against MG. Results: A total of 464 commercial broiler sera samples from 5 different geographical regions were subjected to ELISA. From 464 samples tested, 105 samples were positive in ELISA. Our results indicated that the prevalence of MG in broiler farms was 22.62 % during study period. Conclusion: It can be concluded that MG in broiler breeder farms has potency of great economical losses so detailed study prevalence of MG should be carried out for control and prevention of disease.