Study on Antibacterial Compounds from Methanolic Extract of Bark of Prosopis juliflora (Vilayati babhul)?

This study was aimed at evaluating antibacterial potential of the Prosopis juliflora bark (Vilayati bhabul) in attempt to identify potential natural sources for synthesis of new drug to avoid the growing antibacterial resistance. The peals of bark were extracted by hot methanol by Soxhlet extraction method. Antibacterial activity of crude extract was determined by agar cup method on various human pathogens. Antibacterial susceptibility test of crude extract was performed and it showed promising activity against tested organisms. The crude extract was subjected for HPTLC analysis to separate phyto-compounds such as glycosides, tannins, saponins and alkaloids. The crude extract was subjected to Activity guided fractionation with different polarity solvents, showed varying levels of bactericidal activity. Fraction D and fraction F shows maximum activity. MIC of fraction D was 3.38mg/ml and 1.69mg/ml for Staphylococcus aureus and Escherichia coli respectively whereas MIC of fraction F for these two organisms was observed to be 2.39mg/ml and 4.77mg/ml respectively and also HPTLC and bioautography was performed. Phytochemical analysis was carried out by tube method and HPTLC plate. Preparative HPTLC was performed to obtained semi purified bioactive compound and separated band were subjected to AST, UV spectroscopy, GCMS, FTIR,CHNS(O) analysis and NMR spectroscopy.