Knockdown (Micro-RNA Inhibiting Technique) Technique for Evaluation of Alpha-1a Adrenergic Receptor and P2x1 Receptor Functions

The new approach regarding the knocking down the protein expression by the use of shmRNA complimentary to the mRNA sequence of the respected protein expression have a major impact on the research area. The knockdown of P2X1 and ADRA1A genes have no effect on chromosomal DNA but have a lack of effect on expression of P2X1 and α1A receptors. The produced genetically engineered male mice can be of 100% infertile. The simple PCR (polymerase chain reaction) based approach can be used for the generation of knockdown cell lines. The Animals should have the treatments according to the guidelines mentioned under international society for applied ethnology. The SIRION biotech have shown the managed shRNA knockdown of about 90% significance interval.