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Optimization of Production Media of Novel Phytase from Aspergillus Niger Using Wheat Bran Waste

Journal: International Journal of Science and Research (IJSR) (Vol.3, No. 6)

Publication Date:

Authors : ; ;

Page : 949-951

Keywords : phytase; wheat bran waste; inorganic phosphate; Aspergillus niger; solid state fermentation;

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Phytase, an acid phosphatase enzyme, efficiently cleaves phosphate moieties from phytic acid thereby generating myo-inositol derivatives and inorganic phosphate, was indentified during screening of endophytic fungi Aspergillus niger. Phytase was produced in selective media at room temperature for 3 days. Production media was also optimized varying the concentration of ingredients from 5 to 25g by using solid-state fermentation for 7 days to check the activity of phytase. The zone of inhibition of crude and pure phytase were 2.1 and 2.4cm recorded using well diffusion method.

Last modified: 2014-06-24 20:30:52