Overview of single-molecule long RNA-seq reads and their transcriptomic applications in plant research

Understanding transcriptome regulation and gene functions needs a systematic method to fully unravel the gene expression profiles, including the isoform classification, at both molecule and single-cell levels. Nowadays, alternative splicing and gene-fusion analysis can take advantage of the third-generation sequencing technology such as PacBio ISOSeq and Oxford Nanopore Technologies (ONT) MinION RNASeq [1] strides at the unprecedented speed in plant organs and cells research area.