Age-related oxidative stress in patients with pyelonephritis

The objective of the present study was to compare age-related oxidative stress in pediatric and adult patients with pyelonephritis. Methods. This research is focused on the age features of change of oxidant-antioxidant parameters and intensity of oxidative stress (OS) in the blood of 252 adults and 123 of pediatric patients with pyelonephritis. The control group consisted of 68 healthy donors of the same age. Along with the standard diagnostic methods, we defined the content of malonic dialdehyde in serum and erythrocytes, the content of ceruloplasmin, transferrin and SH-groups in the blood serum, the index of the OS, activity of glutathione reductase, glutathione peroxidase and total peroxidase activity in erythrocyte. Statistical analysis was performed using the programs Statistica 10 for Windows and "MedCalc". Results. Our results confirmed the increase of concentration of malondialdehyde and protein carbonil groups in the blood and decrease of antioxidant markers, namely: concentration of transferrin, different factions of thiol groups, the total peroxidase activity in erythrocyte, and the activity of glutathione reductase and glutathione peroxidase. The increase of the activity of the oxidation of lipids and proteins due to lower indices of antioxidant protection (content of transferrin, thiol groups, thetotal peroxidase activity in erythrocyte, the activity of glutathione reductase and glutathione peroxidase) was characteristic for patients with chronic pyelonephritis. The highest intensity of OS and concentration ceruloplasmin in serum, were observed in the acute pyelonephritis. The highest activity of lipid peroxidation and low antioxidant protection of the blood are characteristic of pediatric patients. Conclusions. Thus, the intensification of OS depends on the age of the patients with pyelonephritis.