Preparation and Characterization of Biodegradable Glimepiride Loaded PLA Nanoparticles by o/w Solvent Evaporation Method Using High Pressure Homogenizer: A Factorial Design Approach

Glimepiride is used for the treatment of type 2 diabetes mellitus, one of the third generation sulfonylurea drugs having poor aqueous solubility, slow dissolution rate and low elimination half life. The Aim of the present study was to prepare Glimepiride loaded poly-D, L-lactide (PLA) Nanoparticles by o/w solvent evaporation method using high speed homogenizer. Prepared Nanoparticles were characterized for the drug content, encapsulation efficiency. Physical state of the drug, polymer and Nanoparticles were determined by Particle size distribution, differential scanning calorimetry (DSC), X- ray diffraction (X-RD), Fourier transform infrared spectroscopy (FTIR). Particle morphology was observed by FE-SEM. The results of this study showed the potential of the O/W emulsion for drug delivery application. Drug content and Encapsulation efficiency were found to be 40.27 and 80.55% respectively and particle size of the Nanoparticles was 442 nm (dnm). DSC and XRD study showed partial interaction between the drug and polymer.