A New Spectrophotometric Method for the Determination of Isoprothiolane in Environmental Samples

A new spectrophotometric method for the determination of fungicide isoprothiolane. The method is based on the bromination of isoprothiolane to form dibromoisoprothiolane which react with Potassium iodide-Potassium iodate mixture in the presence of leucocrystal violet (LCV) to form a violet colored complex which is soluble in 30% alcohol. The complex shows maximum absorbance at 600 nm. Beer’s law obeyed over the concentration range of 5.0-25 ?g in a final solution volume of 10 ml. The molar absorptivity of the colored system is 4.6×104 L mol-1cm-1 and Sendell’s sensitivity is 2.0×10-3 ?g cm-2. The reproducibility assessed by carrying out seven days replicate analysis of a solution containing 20 ?g of isoprothiolane in a final solution volume of 10 ml. The standard deviation and relative standard deviation for the absorbance value were found to be ± 1.33×10-4 and 4.1×10-2% respectively. The proposed method is free from the interference of other toxicants. The analytical parameters were optimized and the method was applied to the determination of isoprothiolane in water, food and human sample.