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Separation and Identification of Many Natural Products from Emblicaofficinalis Fruits and Study of their Antibacterial Activity

Journal: International Journal of Environment, Agriculture and Biotechnology (Vol.3, No. 6)

Publication Date:

Authors : ;

Page : 2192-2197

Keywords : GLC analysis; Emblicaofficinalis; fatty acid; phenolic compounds.;

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The active constituents were separated from Emblicaofficinalis fruit, using column chromatography (CC) with solvent system of (pet.ether:ethyl acetate; 5:1) from petroleum ether extract (A1) and solvent system (chloroform:Methanol; 10:1) from Ethanol extract (A2). Saponification process was carried out on (A1F2) to separate many fatty acids and identification of five fatty acids (Heptanoic, Octanoic, Lauric, Palmitic and also cis& trans Oleic acids) using GLC-analysis. Moreover, acid hydrolysis was also carried out on (A2F2), to separate many free poles of phenolic compounds, such as (Hydroquinone and Gallic acid) were identified using HPLC-analysis. The active separated compounds under study showed different effects against the micro-organisms (m.o), using disc diffusion method turbidity.

Last modified: 2018-12-22 20:41:30