Spectrophotometric Determination of Caffeine in Selected Pakistani Beverages

All reagents used in this study were of analytical or HPLC grade and all solutions were prepared by using disttled water. Caffeine standard was purchased from Sigma-Aldrich (Sigma-Aldrich Chemie GmbH Munich, Germany). A Perkin Elmer (Germany) double-beam UV/VIS spectrophotometer with 1-cm matched quartz cells was used for all absorbance measurements. A pH-meter, Thermo Orion (Thermo scientific fisher, USA), with combined electrodes was employed for measuring pH values. Analytical balance (Kern and sohn, GmbH, Germany) was used for weighing purpose throughout the study. Bath sonicator(Fisher scientific, UK) was used for sonication purposes.