ANTIMICROBIAL EFFECTS ALCOHOLIC EXTRACT OF YARROW (ACHILLE AMILLEFOLIUM) AGENTS STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI THE MOST COMMON SOURCE OF URINARY TRACT INFECTION

Effect plant extract to eliminate resistance genes in E. coli andS.aureuswere isolated from urinary tract infections. All the samples in this research were taken from the pregnant woman with the age of (25-45) in Sulaimani city during May (2015-2016). Out of 200 different urine samples, 50 positive cultures were isolated and diagnosis. In (35) samples, E. coli were the predominant microorganisms and were responsible for (17.5%) of the urinary tract infections and the other 15 samples (7.5%) were S.aureus. All isolates were obtained depending on cultural, morphological identification, in addition to, API20Esystem. Theresistances of the isolateswere examined against eleven widely used antimicrobials, which were Cefotaxime(Cef),Ampicillin(Amp),Nitrofurantoin(Nit), Penicillin(Pn), Trimethoprim(Tri),Rifampicin (Rif), Tetracycline(Tet), Gentamycin(Gm),Streptomycin (Str), Ciprofloxacillin (Cip) andAmikacin(Ak). The positive cultures were classified according to their resistance to the above antimicrobials. They were grouped into (8) E. coli antibiograms and (5) S. aureusantibiogram. All the isolates were resistant to (Pi). Three isolated for E. coli (E3, E18, and E35) and two isolated for S.aureus (S39 and S45), resisted to all the tested antibiotics while the other samples varied in their resistance. The ethanolic extracts inMinimum Inhibitory Concentration [MIC] for Achilleamillefolium was (3000) μg/ml. Sub-MIC of plant extract was used as eliminated antibiotic resistant genes of isolates and the results were SMIC 2500 μg/ml of ethanolic extract of Achilleamillefolium eliminated the genes that are responsible for E. coli (E3, E18, E35) isolates (Ak, Str, Rf, Gm, Tri, Tet, Cip), while reduced the percentage of resistance for these antibiotics Amp, Cef, and Pi which ranged between (100%- -10%), SMIC3500 μg/ml of ethanolic extract of Achilleamillefolium affected on (S39) genes responding on Str, Rf, Amp, Ak, Gem, Nit, Tri, Tet, Cef and Cip reduced the percentage (%100, while not affected byresistantgene in (Pi), although the genes in (S45) Responding on Ak. Str, RF, Gem, Nit, Tri, and Tet while not affected on resistant gene in (P, Cef, Cip, and Amp).