CHEMICAL PROFILING OF BERGENIA CILIATA(HAW.) STERNB. AND INHIBITION OF KEY ENZYMES LINKED TO PRIMARY HYPEROXALURIA TYPE1 (PH1): AN IN VITROAND IN SILICO STUDY

This study sought to assess the inhibitory activities of Bergenia ciliata on oxalate synthesizing key enzymes glycolate oxidase and lactate dehydrogenase in vitro and in silico. Glycolate oxidase and lactate dehydrogenase enzymes were extracted from rat liver and purified by using Ion-Exchange chromatography(DEAE-Cellulose - 52) and characterized. Ethanolic extract of B.ciliata was evaluated in vitro for its ability to inhibit the major enzyme activities of GOX and LDH through spectrophotometrically and mode of inhibition were evaluated using Lineweaver-Burk plots. GOX and LDH inhibitory activities were also figured out with molecular docking analysis. Iso-electric focusing and Sodium Dodecyl Sulfate –Polyacrylamide gel electrophoresis (SDS-PAGE) study revealed enzyme glycolate oxidase with PI value 9 with Molecular weight ~41 KDa and enzyme lactate dehydrogenase with molecular weight ~35 KDa. All extracts showed good amount of free radical scavenging activities. UV-visible analysis and FT-IR analysis confirmed presence of alcohols, phenols, alkanes, alkynes, alkyl halides, aldehydes, carboxylic acids, aromatics, nitro compounds and amines in extracts. GC-MS analysis detected compounds might be accountable for antiurolithiac, hyperoxalouria synthesis enzymes inhibitors and antioxidant nature. Considering the results, drug formulation of investigated plant's lead compounds can be used in remedies for primary hyperoxaluria type1 (PH1) and other renal disorders.