Production and Purification of Pectinase Enzyme from Aspergillus Candidus and its Application on Tea Processing

Enzymes are macromolecular biocatalysts which are commercially used in pharmaceuticals, biofuels, food and beverages and consumer products. The enzyme that hydrolyse pectin substances are called as pectinases or pectinolytic enzyme. The organism such as Bacteria, fungi and yeast are used to produce pectinase enzyme. The pectinase enzyme have been used in several conventional industrial process, such as textile plant fiber processing, tea, coffee, oil extraction, treatment of industrial wastewater. Production of pectinase through micro organism which give more effective and low cost production for industries. In this present research work the spoiled fruits (Apple, citron and Grape) and vegetables (Tomatoes) are collected and extracted. The pectin producing organism is isolated and fermented. Enzyme assay was done for the production of pectinases. The produced pectinase are purified for further application on tea leaf processing