A Novel Treatment of Symptomatic Menopause Autologous Ovarian Transplantation Research in Progress| Biomed Grid

Following the onset of menopause, some women suffer from significant effects, including hot flashes, osteoporosis, mood swings, loss of libido, among others. While natural menopause occurs at the age of 50 plus, on occasion this process may be induced at age 40 or less due to premature menopause [1,2]. Materials and Methods After obtaining informed consent, partial opportunistic oophorectomy was performed laparoscopically or by laparotomy as a part of indicated cesarean section or gynecological operation. After its removal, the ovary was placed in a biohazard bag containing cold, sterile Hanks balanced salt solution. Working in a fume hood and using standard sterile techniques, the ovarian cortex was dissected from the stroma and cut into 1x0.5cm strips. Each strip was placed in a cryovial. The vial was filled with protective solution and equilibrated on a rotating mixer at 4C for 30 minutes. These vials were then placed into a -80C freezer in a styrofoam covered box where the temperature dropped gradually over a 24-hour period. At the end of this period, the specimens were transferred to a cryofreezer cane and placed inside cryoflex tubing. Separate pieces of tissue were sent at times to pathology for light and electron microscopy to ascertain their condition