DENITRIFICATION KINETICS OF PSEUDOMONAS STUTZERI AZ101

Nitrogen removal from environment is significant due to its effect on health and the whole ecosystems. Hence a highly nitrogen tolerant aerobic denitrifier, Pseudomonas stutzeri AZ101 was isolated from aquaculture wastewater and characterized using 16S rRNA analysis. The ability of the isolate to carry out simultaneously nitrification and denitrification (SND) was investigated. Different initial concentrations of nitrate and ammonium were used to determine the rate of SND. The denitrification data fitted well with the second order reaction kinetics with correlation coefficient between 0.923-0.9588. The nitrification data does not fit to both first and second order reaction kinetic but progressive shifts in the logarithm phase of the growth were observed at different initial concentrations. The kinetics data provide a significant insight to nitrogen removal from the wastewater.