Complexation of Andrographolide, Mirabegron and Suvorexant and Bioactivity Study of the Complexes

Background: There are many ways to synthesize new drug molecules for more potent therapeutic efficacy with minimum side effects. The drugs may exhibit therapeutic effects independently or may interfere with each other. Method: In this study, new drug molecules were synthesized by drug-drug complexation way according to the calculated w/w ratio. The complexes were prepared by solid-mixing method using pestle and mortar by adding equal amount of drugs to each other, then warmed at 50 °C for 4 hours and left at room temperature for overnight to obtain new drug complexes. The new drug complexes were distinguished by Differential Scanning Calorimetry(DSC) and Fourier-transform infrared spectroscopy (FTIR) with the biological activity. Results: The melting endothermic peaks of andrographolide-suvorexant, andrographolide-mirabegron, and suvorexant-mirabegroncomplexes were found at 221.02 °C, 189.49 °C and 115.78 °C, respectively which were different from the melting endotherms of andrographolide, suvorexant and mirabegron at 230.87 °C, 127.09 °C and 140.40 °C, respectively. The FTIR spectra of the formed complexes showed the similar characteristics of the standard drugs. The cytotoxic effect of the drug complexes on HeLa cell line were higher (10% less survived HeLa cell, i.e. > 90 % cell died) than the precursor drug molecules. Conclusion: Our research findings that three new drug compounds were synthesized by complexation way which could be due to interaction of andrographolide and suvorexant; andrographolide and mirabegron, and suvorexant and mirabegron.