INDIRECT ORGANOGENESIS IN SOLID AND LIQUID MEDIUMS FROM VITROPLANTS OF AGAVE TITANOTA

In vitro cultivation is a useful technique for study, improvement and conservation of agaves. It was used as explants pieces of leaves and roots in MS solid and liquid mediums respectively, supplemented with Naphtaleneacetic Acid (NAA by its abbreviations in English) Benzylaminopurine (BAP by its abbreviations in English) and Dichlorophenoxyacetic Acid (2,4-D by its abbreviations in English) It was used concentrations of 0.0, 0.5, 1.0 and 2.0 mgl-1 to induce shoot formation. It was reported the development of callus within one month and half on solid cultivation, shoot formations were presented using 2.0 mgl-1 BAP and 0.5 mgl-1 of NAA having 60?1 shoots each container with hydration aspect, meanwhile in the combination 1.5 mgl -1 of BAP with 0.5 mgl-1 of NAA a lower number of shoots was reached, but they were healthier. The formation of shoots in liquid medium was presented within a month of cultivation, obtaining 6.6?1 shoots by healthy explants, even in the control treatment. Embryogenic callus was generated using BAP and 2,4-D.