EXAMINATION OF ANTIMICROBIAL PEPTIDES FROM SERROGNATHUS TITANUS LARVAE

This study was done to investigate the antimicrobial activity of stag beetle larvae extract induced by innate immune response by treating Salmonella strain. Agar diffusion assay showed no significant difference in antimicrobial activity compared to the control. However, when the activity was done by microtiter plate assay, the antimicrobial activity was increased compared to the control. The antimicrobial activity was measured according to the molecular weight to confirm that the activity was induced according to the molecular weight of the peptide. As a result, many antimicrobial substances against S. Gallinarum were found at below 3 kDa and above 30 kDa. Many antibacterial substances against S. Enteritidis were observed at below 3 kDa, 10-30 kDa and above 30 kDa. Therefore, it is suggested that stag beetle larvae induce an antimicrobial peptide, which is an innate immune response, against pathogenic microorganisms.