POLYMORPHISM OF XPD GENE IN COLORECTAL CANCERJournal: NAUKA MOLODYKH (Eruditio Juvenium) (Vol.7, No. 3)
Publication Date: 2019-09-30
Authors : E.P. Kulikov S.A. Mertsalov A.A. Nikiforov A.I. Sudakov V.A. Grigorenko;
Page : 340-348
Keywords : colorectal cancer; gene polymorphism; DNA repair genes;
Aim. To assess the polymorphism of XPD gene in patients with colorectal cancer. Materials and Methods. Evaluation of gene polymorphism was conducted in a group of 143 people. The main group consisted of 105 patients with colorectal cancer who were examined and treated in the Ryazan Regional Oncological Dispensary in 2016-2018. The control group of 38 people consisted of healthy volunteers. Genotyping was performed by DNA extraction from leukocytes of venous blood of the patients with subsequent PCR with electrophoretic detection of the result. Results. The statistical analysis revealed the absence of a reliable association of XPD gene polymorphism with age and gender, both within and between the groups (p>0.05). Comparison of the groups for the frequency of detection of different variants of XPD gene genotype revealed predomination of heterozygous form of the gene in the main group, while in the control group the heterozygous form was in the same ratio with the homo1 form. However, these differences were not statistically significant (p>0.05). There was statistically significant difference in XPD gene polymorphism in groups of female patients aged 60-69, where the heterozygous form of the gene prevailed in comparison with the control group of the same age where the homo1 form prevailed (p=0.017), and in the main group of patients aged 70-79 of both genders with a control group of the same age, where similar ratios of phenotypes were observed (p=0.039). In the group of male patients aged 70-79 the absence of the homogeneous form was observed (p=0.042). The association of XPD gene polymorphism with the stage and localization of the tumor process was not statistically significant (p>0.05). Conclusions. The gender and age criteria did not affect the polymorphism of XPD gene. XPD gene polymorphism showed reliable differences in the group of female patients aged 60-69, patients aged 70-79 of both genders and male patients aged 70-79 as compared with the group of healthy volunteers. Localization and stage of colorectal cancer was not associated with XPD gene polymorphism. A further study of the polymorphism of XPD gene in colorectal cancer appears to be promising from positions of its connection with the course of the disease in patients of the older age group.
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