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Selection of Lactic Acid Bacteria (LAB) Origin of Food Fermentation Probiotic Mixed as Candidate for Broiler

Journal: International Journal of Environment, Agriculture and Biotechnology (Vol.5, No. 1)

Publication Date:

Authors : ;

Page : 68-75

Keywords : Lactic Acid Bacteria; Pathogenic Bacteria; Bile Salts; pH 2.5; Probiotics.;

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This research aims to looking for 2 candidates from the new probioticlactic acid bacteria fermented food origin. This study uses seven isolates were isolated from whey, cassava, and fish budu. 7 isolates have been tested potential as a producer of glutamic acid but not known as a candidate probiotic petensinya. This study uses a completely randomized design, each repeated 3 times with 5 stages of the research are: 1) Testing capability isolates at low pH (pH 2.5); 2) testing the ability of isolates in bile (0.3 and 0.5%); 3) Viability isolates 4) Testing capability isolates in killing the pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Salmonella enteritidis). The results showed the 7 isolates can be used as a probiotic candidates with the results of the resistance to pH 2.5 for 3 hours is 63.91-92.75% and the 6 hour incubation is 48,76-72.44%, resistance to bile salt concentration of 0.3% showed resistance to 22.86-83.57% and a concentration of 0.5% showed resistance 18.35-72,77%, 81.05-92.67% viability and inhibitory effect on the bacteria Escherichia coli is 7:33 to 12:01 mm, is 11:11 to 14:05 mm Staphylococcus aureus and Salmonella enteritidis is 12:02 to 18:08 mm. From 7 isolates after penseleksian ability as a candidate probiotic mixture then isolate F6 from durian fermented and isolate C8 from buffalo milk fermented (Dadih) are able to live at pH 2.5 for 3 hours of 92.75% and 86.06%, at 6 hours of 72.44 and 71.42%, the bile salt 0.3% were 83.57% and 78.75% and the concentration 0.5% are 77.17% and 72.77%, with viability were 92.67% and 92.23%, while the ability to kill pathogens such as Escherichia coli was 10.49 and 8.89 mm, Staphylococcus aureus was 14.05 and 13.70 mm, dan Salmonella enteritidis was 18.08 and 14.18 mm.

Last modified: 2020-02-14 02:45:44