NOVEL HPLC METHOD FOR DETERMINATION OF POLYPHENOLS IN AGRO-INDUSTRIAL WASTE OF DATE FRUITS

Phoenix dactyliferais medicinally important plant. Fruits are traditionally used for nutritional purpose. Fruits and seeds are the rich sources of phenolic acids which exhibits strong antioxidant property. The aim of the work was to develop and validate a novel, simple method for quantification of gallic acid, protocatechuic acid, vanillic acid and syringic acid by High-Performance Liquid Chromatography (HPLC) in seeds of Phoenix dactylifera. The chromatographic analysis was performed on HPLC Shimadzu LC Prominence-i2030 model consisting of UV detector and auto sampler using Cosmosil C18 (250?4.6mm, 5μ) column. The mobile phase used was 0.2% ortho-phosphoric acid: methanol (80:20).The hydroalcoholic extract was quantified by using HPLC-UV. The total run time was 30 min and retention time of gallic acid, protocatechuic acid, vanillic acid and syringic acid was found to be 4.8, 8.7, 20.2, 25.2 min respectively. The analytical method was validated according to ICH guidelines including parameters specificity, linearity and range, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ).Phoenix dactylifera seeds are the rich source of phenolic acids which attribute to antioxidant properties of Phoenix dactylifera seeds. The seeds being agro-industrial waste of date fruits are cheaper source and can be used in medicinal and nutritional areas due to the presence of bioactive phytoconstituents.