DEVELOPMENT AND VALIDATION OF A U.V SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF CISPLATIN IN PHARMACEUTICAL FORMULATIONSJournal: Indian Drugs (Vol.51, No. 7)
Publication Date: 2014-07-28
Authors : Shrikhande S.S. Jain D.S. Athawale R.B. Bajaj A.N.;
Page : 23-30
Keywords : email@example.com;
A simple, accurate, specific and reproducible colorimetric method was developed for cisplatin in pharmaceutical formulations. The analytical method was based on the formation of a stable, light blue coloured complex upon reaction between cisplatin and ortho-phenylene diamine at pH 7.4. The λmax of the solution was obtained at 702 nm in phosphate buffer saline 7.4. Optimized OPDA concentration and complexation time for the procedure were 1.2 mg/mL and 10 minutes respectively. Developed method in PBS 7.4 was found to be linear in the range of 1-10μg/mL with a regression coefficient of 0.999 and exhibiting line equation y = 0.102x – 0.004. Recovery of marketed cisplatin injection was found to be in the range of 98.8-99.1%. Drug content for the developed nanoformulation was in the range of 98-101% indicating no interference of excipients. These results illustrate that the developed method could be successfully employed for quantitative estimation of cisplatin in pharmaceutical formulations.
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