A NEW RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF FLOUXAMINE, CHLOMIPRAMINE HCL IN ITS BULK AND PHARMACEUTICAL DOSAGE FORMSJournal: Indo American Journal of Pharmaceutical Sciences (IAJPS) (Vol.1, No. 3)
Publication Date: 2014-10-08
Authors : Vinod Kumar.Gongalla; K. Vanitha Prakash; Ravi Pratap Pulla;
Page : 163-170
Keywords : Flouxamine; Chlomipramine Hcl; RP-HPLC; Methanol and Acetonitrile;
The objective of the current study was the development of a simple, precise and accurate isocratic reversed-phase High Performance Liquid Chromatography [RP-HPLC] assay method and validated for determination of Flouxamine and Chlomipramine Hcl in capsule dosage forms. Isocratic separation was achieved on a C18 Inertsil ODS (5μ, 250mm× 4.6mm) with flow rate of 1.0ml/min using UV detection at 230nm. Different mobile phase compositions of pH 3.5 phosphate buffer: ACN (30:70) in order to determine the best conditions for the effective separation and elution of the analytes. The mobile phase consisting of pH 3.5 buffer: ACN (30:70) was selected. The injection volume was 10.0 μl and the detection was carried out at 254 nm by using photo-diode array detector. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The proposed method was successfully used to determine the drug content of marketed formulation.
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Last modified: 2014-10-08 17:57:17