Serotyping and Molecular Detection of Diphasic and Monophasic S. typhimurium Isolated from Fresh Chicken Samples Collected from Different Local Markets in Najaf Province during April to October 2012

Salmonella typhimurium is one of most importance serovars associated with human disease reported from poultry in developing countries and during the last few years Monophasic variants of Salmonella Typhimurium-like strains, lacking the fljB-encoded second phase H antigen, appear to be of increasing importance in infection in many Member States such as France, Germanyand but research studies in Iraq did not or rare referred to monophasic variants. Therefore a total of 1440 fresh chicken samples were collected from local markets in Najaf city during April to October 2012 to detect typical diphasic and monophasic S. typhimurium distribution. The results appeared the numbers and percentages of monophasic S. Typhimurium isolates in gizzard, liver and meat were 28 isolates (5.83%), 10 isolates (2.08%) and 3 isolates (0.6%) respectively, when it in typical diphasic S. typhimurium raised to 128 isolates (26.66 %), 71 isolates (14.79%) and 22 isolates (4.5%), respectively, hence the severity of monophasic S. typhimurium isolates in this study may be less than it in studying typical diphasic S. typhimurium isolates.