EVALUATION OF ALKALINE PHOSPHATASE ACTIVITY BY CRYSTALLOGRAMS OF PATIENTS TAKING STATINS

Background. Crystallography of saliva is one of the actively developing scientific fields. Non-invasiveness and low cost of the method make it appropriate for use in screening studies and monitoring the treatment of diseases. Currently, mortality from cardiovascular diseases takes the first place among the causes of death, which justifies the use of statins as the most effective means of preventing these diseases. At the same time, up to 30% of cases of taking statins are accompanied by the development of hepatitis, which is an indication for stopping the use of these drugs. Considering that alkaline phosphatase (ALP) is an indicator enzyme for liver damage and, at the same time, an enzyme actively involved in the exchange of calcium, which determines the structure of saliva crystals, the aim of this study was to develop a method for monitoring ALP activity by the form of saliva crystals in patients taking statins. Material and methods. A total of 22 men who took and did not take atorvastatin were examined. Blood and saliva were taken from the subjects in the morning on an empty stomach. The activity of ALP in blood was determined by a photometric method on the SOLAR CM2203 spectrophotometer. Saliva was crystallized by the S.P. Leus' method. The shape of the crystals was studied using a Leica DM 2000 microscope. Results. It was shown that in individuals taking statins, the activity of ALP was 33.4% lower than in those of the control group and correlated with various forms of saliva crystals. Conclusions. A method for assessing the activity of alkaline phosphatase by crystallograms of saliva with 94.12% accuracy, 100% sensitivity and 88.89% specificity has been developed.