CHARACTERIZATION OF ANTIBODIES FROM EGG YOLK OF SOME BIRDS

The objective of the study on IgG antibody in Egg yolk of some birds, is to characterize by comparing and contrasting, the IgG antibody precipitated from the egg yolk of immunized bird (chicken) and local birds (chicken and Guinea fowl).IgG antibody from the egg yolk of the birds, were purified by ethanol fractionation. The purity of the antibody was monitored by SDS-PAGE and Immunoelectrophoresis, and the immunological activity was tested by Ouchterlony technique. Protein concentrations of the egg yolk from immunized chicken, local chicken and Guinea fowl were 0.87 mg/ml, 2.37 mg/ml and 0.29 mg/ml respectively, while the molecular weights were 70,598, 70,598 and 74,405 KD. From thefigures obtained, a conclusion was drawn that egg yolks of immunized bird and local birds are good sources of immunological active IgG antibody.