A Genetic Variability Study of Lipase Producing Gene among Aeromonas Species Isolated From Clinical Isolates by RAPD DNA Markers

Lipases are most commonly used enzymes and do have lots of industrial applications. Even though these enzymes are used abundantly, little work was done in isolating the enzyme from microbes. Aeromonas species was the common contaminant of nosocomial infections, which do processes this enzyme. Study of such enzymes and their phylogenetic analysis could reveal loads of information of the organisms applications. We isolated and purified the enzyme from Aeromonas species, from clinical samples. PCR amplification of lipase gene was done to confirm the organism ability to produce lipase. Lipase enzyme was then studied for stability at varying temperatures and pH. The DNA was then used for the RAPD profiling to screen phylogenetic relation. Rhodamine plate assay was used for initial screening of bacteria. Among the 20 isolates screened all of them are lipase positive and only 5 isolates were found to be producing high lipase content. The enzyme was found to be stable at pH 6 and at temperature 27.50C. The phylogenetic analysis revealed that the sequences match to Aeromonas salmonicida, Aeromonas hydrophila, Aeromonas veronii, Aeromonas enteropelogenes and Aeromonas caviae. All the species belong to the same family. This study could pave ways to elucidate and characterize the molecular pathogenesis associated with the lipase gene whch eventually could lead to screening antibacterial targets and novel drugs against many antibiotic resistant nosocomial infections.