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The Application of Modern Biotechnology in Protein Interaction Research-A Review

Journal: Journal of Agriculture and Crops (Vol.7, No. 2)

Publication Date:

Authors : ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ;

Page : 39-47

Keywords : Protein interaction; Biotechnology; Theory; Application; New progress.;

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Intermolecular interaction is the material basis for cells to achieve their functions, and protein-protein interaction is an important approach to illuminate the regulation network of biological molecules and has important theoretical significance and potential application value for revealing the activity law of life in nature. This paper mainly summarizes and analyzes the new advances and applications of modern biotechnologies in the study of protein-protein interactions, including local surface plasmon resonance (LSPR), yeast two-hybrid, GST-Pull-down, bimolecular fluorescence complementation, and co­immunoprecipitation. At the same time, the principles of different research methods for protein-protein interaction and their other applications in the field of life sciences are also discussed, all of these will provide a reference value for the analysis of protein-protein interaction and the molecular regulation mechanism of biomacromolecules.

Last modified: 2021-03-03 19:26:48