Green Synthesis, Characterization and Evaluation of In-vitro Antioxidant Anti-diabetic Activity of Nanoparticles from a Polyherbal formulation-Mehani

Green synthesis of silver and iron nanoparticles was carried out using Polyherbal formulation Mehani and characterized through UV-Visible absorption spectroscopy, FTIR, XRD and SEM respectively. The color change and strong peak at 465nm on UV-spec analysis initially conforms the synthesis of silver nanoparticles. Peaks were not obtained for iron nanoparticles since it is zero valent in nature. The FTIR study reveals the phytochemicals responsible for the reduction of silver and iron nanoparticles. The crystalline nature of the presence of silver and iron nanoparticles was confirmed by XRD analysis by comparing with standard JCPDS file No. 89-3722, 75-0033. Surface morphology and size were identified using SEM analysis and it was found to be 60-80nm, 40-60nm respectively. Total phenol and flavonoid content were also estimated for the synthesized nanoparticles and found to be 2.91 and 1.93 (AgNPs), 3.71 and 5.185 (FeNPs). Antioxidant activities like DPPH, Nitric oxide, H2O2 scavenging activities and reducing power assays were performed. Anti-diabetic activity was performed using alpha amylase inhibition assay and the percentage of inhibition was found to be 65.45% (AgNPs), 70.48% (FeNPs) and 73.87% for standard ascorbic acid. The result clearly indicates that FeNPs possesses better antioxidant and anti-diabetic activity that can be used for the efficientfor the management of hyperglycemia.