A STUDY ON STATUS OF URINARY HYDROXYPROLINE IN POST MENOPAUSAL WOMENJournal: International Journal of Advanced Research (Vol.9, No. 03)
Publication Date: 2021-03-17
Authors : Lalrintlingi Sailo Apurba Sarkar; Suresh Babu;
Page : 136-146
Keywords : ;
Introduction: WHO and the Stages of Reproductive Aging Workshop have defined menopausal transition as the time of an increase in follicle-stimulating hormone and either increased variability in menstrual cycle length, two skipped menstrual cycles with 60 days or more of amenorrhea, or both. It concludes with the final menstrual period. Post-menopause begins at that time, although it is not recognized until after 12 months of amenorrhea. During menopause, women face various physiological, psychological, and biochemical changes. Laboratory medicine has given a new background to overcome the clinicianÃƒÂƒÃ¢Â€ÂšÃƒÂ‚s diagnostic dilemma. Hydroxyproline is mainly found in collagen and accounts for 13% of total amino acid content and derived from proline by post-translational hydroxylation. Hydroxyproline is derived from another amino acid such as proline. Direct urinary assay of hydroxyproline to measure bone resorption have clinical applications as part of screening programs to assess the risk of osteoporotic fractures. Method: A total of seventy patients with regular medical follow-up records, The Patients were pre and post-menopausal women (35 each) recruited for this study. Patient details like body mass index, education, smoking, alcohol intake, dietary habits, and family history were considered before selecting the patients. Analytical Methods: Urinary Hydroxyproline and Urinary creatinine was estimated by Modified Neumann et al and Spectrophotometric JaffeÃƒÂƒÃ¢Â€ÂšÃƒÂ‚s reaction respectively. Result: The study population consisted of 70 participants of premenopausal (n=35) and postmenopausal women (n=35), mean age of 38.11 ÃƒÂƒÃ¢Â€ÂšÃƒÂ‚Ã‚Â± 4.3 and 54.40 ÃƒÂƒÃ¢Â€ÂšÃƒÂ‚Ã‚Â± 4.6 respectively. The bone mineralization marker urinary total hydroxyproline was quantified in pre and post-menopausal women, which is 80.3 ÃƒÂƒÃ¢Â€ÂšÃƒÂ‚Ã‚Â± 75mg/L and 136 ÃƒÂƒÃ¢Â€ÂšÃƒÂ‚Ã‚Â± 103mg/L respectively. The urinary creatinine level in pre and post-menopausal women was 53.7 ÃƒÂƒÃ¢Â€ÂšÃƒÂ‚Ã‚Â± 14.2mg/dL and 37.0 ÃƒÂƒÃ¢Â€ÂšÃƒÂ‚Ã‚Â± 27.3 respectively. The hydroxyproline: creatinine ratio (HCR) was 41% to 69% against the normal reference interval in pre and post-menopausal women. Conclusion: The obtained normative data for the premenopausal woman population would be a new reference range in Indian sub-population or otherwise general population normative reference range commonly being used as a reference interval in all kind of pathophysiological disorders. Hence, the derived parameter confirmed that HCR is the most prognostic significant diagnostic marker in pre and post-menopausal patients.
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