ANTIBACTERIAL EFFICACY OF METALLIC NANOPARTICLES OF CARICA PAPAYA LEAF EXTRACT ON THE CARBAPENEM RESISTANT NDM-1 BACTERIA

The prevalence of multidrug-resistant bacteria causing significant important communicable diseases had turned out to be a matter of great public health concern internationally involving India. Extensive use, availability of practically all the antibiotics over the counter as well as incompletion of the dose are among few reasons that have left clinicians with fewer treatment options. New Delhi metallo-β-lactamase 1 (NDM-1) generating bacteria were averse to almost all β- lactam antibiotics including carbapenems. Lately, biogenic metallic nanoparticles had shown their advantages averse to such MDR microorganisms. So the aim of present research has been to use the combination of green synthesized metallic nanoparticles of plant extract as an alternate to the conventional treatment against antibiotic resistance. The synthesised Zinc nanoparticles of aqueous along with ethanolic extracts of the leaves of Carica papaya were characterized by using UV-visible Spectroscopy, FT-IR also zeta potential. The absorbance of CPL ZnNP(Aq) & CPL -1 ZnNP(Eth) was observed at 397nm and 408 nm respectively, whereas the IR spectrum showed band at 3600-3700 cm -1 and 3566-3700 cm respectively. The zeta capacity has been discovered to be −19.5 mV and −12.6 mV respectively, clearly revealed that the synthesized nanoparticles were stable. The MIC was found to range from 500 to 600 µg/ for both the extracts respectively. The crude extract as well as the synthesized nanoparticles showed good antibacterial activity against the NDM-1 toils of E. coli and K. Pneumoniae. Among all, the aqueous extract indicated additional activity than the ethanolic extract especially against NDM-1 K. Pneumoniae.