Morphological and Molecular Identification of Nutmeg (Myristica Sp) in West Halmahera Using The Barcode MatK Gene

The morphological and molecular identification of nutmeg (Myristica sp) in West Halmahera district using the matK gene has been carried out. The purpose of this study was to determine the morphological and molecular characters of nutmeg (Myristica sp) with matK markers from West Halmahera. The method used is in three stages, namely a field survey for observations, data collection, analysis of morphological diversity and molecular analysis using the matK gene DNA marker. Molecular data analysis was performed with MUSCLE on the Geneious V5.6 program identified in the BOLD system (www.boldsystems.org). The results showed that morphologically the nutmeg (Myristica sp) in West Halmahera has different morphological characters and diversity of around 5 kinds, namely small yellow mace nutmeg (RDKK), large yellow mace nutmeg (RDKB), red mace nutmeg. oblong (RDML), red mace nutmeg (RDFM), and large red mace nutmeg (RDMB). The results of molecular analysis through sequencing showed that the matK gene had a DNA length of 837 base pairs. Nutmeg (RDKK) and (RDKB) are 100 % identical to Sangihe nutmeg (TTandBK) and Indian Nutmeg (KT445278.1-India), in 837 nucleotide base sequences, whereas nutmeg (RDKB) is 98.3 % identical with different nucleotide sequences in 13 (A-C), 16 (G-A), 17 (G-T), 18 (A-G), 19 (T-G), 812 (T-A) and 834 (T-A), 98.8 % identical nutmeg (RDFM), different in nucleotide sequences 11 (C-T), 12 (T-C), 14 (C-T) and 23 (A-C), nutmeg (RDMB) is identical 99.9 % different on the order of 834 (T-A). Samples with morphological characters of nutmeg with yellow mace were M.fragrans. The morphological characteristics of nutmeg with red mace, both large and small, need further research.