Phytochemical Screening, Total Phenolics, Total Flavonoids and Antioxidant Activity of Talas Steam Ethanol Extract (Colocasia esculenta (L.) Schott)

Talas stems (Colocasia esculenta (L. ) Schott) are known to have antioxidant activity. Extraction of talas stems used maceration method with 96 % ethanol. Determination of total phenolic content used the Folin-Ciocalteu method with a gallic acid comparison compound. Whereas the colorimetric method was used to determine total flavonoid content with 10 % AlCl3 reagent and 5 % acetic acid. The antioxidant activity test applied DPPH (1, 1-diphenyl-2-picrylhydrazyl) method with a quercetin comparison compound. The series of ethanol extract of talas stem were 20, 40, 60, 80 and 100 ppm. The comparison of quercetin was 2, 4, 6, 8 and 10 ppm. The results of total phenolic levels obtained were 78.98 mg GAE / g extract, total flavonoid levels were 97.38 mg QE / g extract and IC50 results of talas stem extract were 74.75 ppm, quercetin was 5.96 ppm. Based on the level of antioxidant strength, quercetin had very strong antioxidant activity (IC50 less than50 ppm), because quercetin was a pure compound. While talas stem extract had strong antioxidant activity (IC50 50-100 ppm).