The Combination Effect Analysis of Catharanthus roseus, Abelmoschus manihot and Dysphania ambrosioides on Rattus norvegicus Blood Triglyceride Content

In the Pandemic Covid-19 era, the search for herbal medicines for community use became the hope of researchers. Triglycerides in the blood are needed by the body at certain levels, but it is a problem at excessive levels or called hypertriglyceridemia. The study aims to analyse the combination effect of the Tapak dara (Catharanthus roseus), Gedi (Abelmoschus manihot) and Pasote (Dysphania ambrosioides) on the blood triglyceride levels of white rats (Rattus norvegicus). The research method used is experimental. This method is carried out gradually starting with the maintenance of rats and administering fats, making extracts, administering treatment, and blood trafficking mice. The research uses samples of Tapak dara, Gedi and Pasote. The dried sample is then extracted by brewing simplisia in the form of tea using boiling water 100 mL, then administered to rats by way of Sonde/Cekok for 30 days. Rat blood after treatment is taken from the heart and then analyzed its triglyceride levels with an enzymatic spectrometer. Results of analysis of the influence of the combination of Tapak dara, Gedi and Pasote show distinct results. After further testing with Duncan's test that the Gemfibrozil treatment resulted in 17.2 mg/dL triglyceride levels and the highest was the third combination treatment of 112.7 mg/dL. The visible effect is the decrease and increase in triglyceride levels. The conclusion is that there are different influences from the combination administration of Tapak dara, Gedi and Pasote against the blood triglyceride levels of white rats with uncombined treatment. Triglyceride levels given the combination of the third leaf extract (PTG) have increased, while in Gedi treatment only and the combination of Tapak dara Pasote is decreased. The combination of several medicinal plants produces a new effect which is the interaction of the effect of a single active compound.