Bacterial Diversity from Bottoms Tanks

Bioremediation of crude oil contaminated soil is an effective process that makes use of ubiquitously microorganisms distributed in the bottom tank samples. In this study, 20 crude oil degradating native bacteria were isolated from a bottom tank sludge sample from oil industry after a treatment with solvent 1: 1: 1 (Bottom tank sludge: diesel oil: water). Bacterial count and identification were done by Sherlock MIDI method, finding Arthrobacter oxydan, Bacillus sp. , Bacillus-simplex, Bacillus sphaericus, Paenibacillus polymyxa, Staphylococcus cohnii cohnii. All microorganism has grown in presence of hexadecane, ciclohexane, kerosene and diesel oil.