Administration of Papaya (Carica Papaya Linn) Seeds Extract Cream 40% Decrease MMP-1 Expression and Increase Dermal Collagen of Male Wistar Rats (Rattus Norvegicus) Exposed to UV-B Ray

Background and purpose: Exposure to UV-B may induce free radicals in the form of ROS, which cause oxidative stress on the skin. This will trigger the activation of AP-1 (activator protein-1) which will induce matrix metalloproteinase (MMPS) especially MMP-1 so that it can degrade dermal collagen and cause photo aging. Papaya seeds contain antioxidant compounds namely flavonoids (quercetin), tannins, and phenols. Flavonoids can prevent an increase in MMP-1 by inhibiting phosphorylation of MAPK (mitogen-activated protein kinases). The purpose of this study was to proof whether giving papaya seeds extract cream 40 % decrease MMP-1 expression and increase the amount of dermal collagen in male Wistar rats exposed to UV-B ray. Methods: Experimental research was conducted with a randomized posttest only control group design. Subjects were white male Wistar rats (Rattus norvegicus).36 animals were divided into two group (n=18 individuals). The control group (mice were given a cream base and were exposed to UV-B). The treatment group (rats were given a 40 % Bali papaya seed extract cream and exposed to UV-B rays). The total UV-B dose given was 840mJ/cm2 for 4 weeks. After 28 days of treatment, MMP-1 levels were examined using the immunohistochemistry method from average of three microscope field of view while the amount of dermal collagen were examined digitally by calculating the average percentage of three microscope field of view of collagen pixels that appear in the histopathological preparations using Picro Sirius Red staining. Results: The comparative analysis results of independent sample t-test showed the mean MMP-1 expression of the control group was higher (27, 46 4, 29 %) compared to treatment group (9, 72 3, 46 %) (pless than0, 001). While the mean amount of collagen in the control group was lower (57, 82 3, 05 %) compared to the treatment group (82, 40 2, 62 %) (pless than0, 001). Conclusion: Based on the results of this study it can be concluded that the cream of Bali papaya seed extract 40 % (Carica papaya linn) decreased MMP-1 expression and increased of dermic collagen on skin of male Wistar rats (Rattus norvegicus) exposed to UV-B rays.