ESTIMATION OF ALOSETRON HYDROCHLORIDE IN TABLET DOSAGE FORM BY RP-HPLC

A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Alosetron Hydrochloride (AST) in tablet dosage forms. Waters Spherisorb® 5μm CN, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase consisting of 0.01M Ammonium Acetate in water adjusting the pH-3.2 with Methanol & Tetra Hydro Furan in ratio of 700:240:60 v/v & mobile phase used as a diluent in the gradient mode. The flow rate was 1.0 ml/min and the effluents were monitored at 295 nm. The retention time was 11.570 min and the detector response was linear in the concentration range of 56.1- 673.2 μg/mL for AST successively. The respective linear regression equation being Y= 27228.755x + 221036.4041 for AST. The Limit of Detection (LOD) & The Limit of Quantification (LOQ) was found to be 0.1 & 0.3 μg/mL for AST. The percentage assay of AST was 99.93%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of AST in bulk drug and in its pharmaceutical dosage forms. .